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New application of fluorescence probe to detect apoplastic redox radicals from plant roots were sought. This probe can detect radicals selectively. Calibration curve for radicals was obtained using nitrogen monoxide as radical standard produced by NOC7. Apoplastic radicals released constitutively were quantified and the release rate was 60 μmol L−1 h−1. Oxidative burst triggered by chitin was distinguished from constitutive radical release.

A new application for the quantification of apoplastic redox radicals of plant roots using pre-fluorescent probe