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Understanding the long-term effects of land use on soil organic carbon (SOC) is of interest due to the possibility of using soils as carbon (C) sinks. We determined how the C levels in the labile (C 0 ) and stable (C r ) fractions of the organic matter of soils of the Pampas were affected by land use, applying the inverse analysis methodology. Soil samples (0–50 cm) taken from 198 sites under different land uses (forest, grassland, and cultivated soil) were incubated for six months, adjusting a one pool exponential model to C mineralization data. The model allowed a good estimation of C 0  (R 2  = 0.96–0.98) and C r  was calculated by difference with SOC. The stocks of C 0  and C r  were calculated on an equivalent mass basis (5000 t soil ha −1 ) for comparisons. About 80% of the SOC is found in the C r  pool in the Pampas soils. Both C 0  and C r  were higher in forest and grassland than in cultivated soils. Soil cultivation produced an average reduction of 30% of the C 0  pool and 18% of the C r  pool in relation to grassland soils. Consequently, most of the SOC lost due to agriculture came from the C r  pool. In mixed production systems, no differences of the C pools were detected between the crop or pasture phase of the rotation. The parameters C 0  and k (mineralization rate constant) of the exponential model were negatively correlated, so the comparison of C 0  between different land uses must be careful. The k parameter was similar among land uses when the comparison was made for similar values of C 0,  suggesting that land use affected the size of the C pools not their stability. Forest and grasslands were effective systems for sequestering C in stable forms. Expanding the cultivated area in the Pampas should be avoided if it is desired not to emit C into the atmosphere from the soil.

Land Use Effects on Soil Organic Carbon Fractions in the Pampas of Argentina Determined via Inverse Analysis of Carbon Mineralization Data