Open AccessHuman DNA topoisomerase I is encoded by a single-copy gene that maps to chromosome region 20q12-13.2.
Author(s) -
Chung-Ching Juan,
Jaulang Hwang,
Angela A. Liu,
Jacqueline WhangPeng,
Turid Knutsen,
Kay Huebner,
Carlo M. Croce,
Hui Zhang,
James C. Wang,
Leroy F. Liu
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.23.8910
Subject(s) - biology , complementary dna , microbiology and biotechnology , gene , topoisomerase , dna , chromosome , genetics , southern blot , human genome , metaphase , chromosome 22 , nucleic acid thermodynamics , genome , rna
cDNA clones of the human TOP1 gene encoding DNA topoisomerase I (EC 5.99.1.2) have been obtained by immunochemical screening of phage lambda libraries expressing human cDNA segments, using rabbit antibodies raised against purified HeLa DNA topoisomerase I. Hybridization patterns between the cloned cDNA sequences and human cellular DNA and cytoplasmic mRNAs indicate that human TOP1 is a single-copy gene. The chromosomal location of the gene has been mapped to the long arm of chromosome 20, in the region q12-13.2, by hybridization of a radioactively labeled TOP1 cDNA probe to human metaphase chromosomes and to a panel of rodent-human somatic hybrids retaining overlapping subsets of human chromosomes.