Open AccessQuantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker.
Author(s) -
Thomas R. Skopek,
Howard L. Liber,
John J. Krolewski,
William G. Thilly
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.1.410
Subject(s) - salmonella , mutation , microbiology and biotechnology , chemistry , biology , genetics , bacteria , gene
We have developed a quantitative forward mutation assay using Salmonella typhimurium, in which resistance to the purine analog 8-azaguanine is used as a genetic marker. We present the assay protocol, the concentration-dependent toxicity and mutagenicity of five known mutagens (N-methyl-N'-nitro-N-nitrosoguanidine, ICR-191, 9-aminoacridine, dimethylnitrosamine, and benzo[a]pyrene), and reconstruction experiments testing the assay for possible bias. The relative merits of forward versus reverse mutation assays are discussed.