Open AccessAn assay of ribonuclease H, endoribonucleases, and phosphatases.
Author(s) -
J G Starvianopoulos,
Erwin Chargaff
Publication year - 1976
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.5.1556
Subject(s) - ribonuclease , biochemistry , enzyme , phosphatase , rna , chemistry , phosphodiesterase , dna , moiety , substrate (aquarium) , microbiology and biotechnology , biology , stereochemistry , gene , ecology
An assay for ribonuclease H (EC 3.1.4.34) is described which permits the estimation of the number and the type of cleavages produced by the enzyme when acting on the ribo moiety of a DNA-RNA hybrid substrate. With six different homopolymer hybrids tested, the number of enzymically induced breaks varied widely. The method is applicable to other endoribonucleases, phosphodiesterases, and phosphatases.