Open AccessInactivation of Ribosomes In Vitro by Colicin E 3
Author(s) -
Thierry Boon
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.10.2421
Subject(s) - colicin , ribosome , in vitro , in vivo , rna , protein biosynthesis , incubation , biology , escherichia coli , biochemistry , microbiology and biotechnology , chemistry , genetics , gene
Cell-free protein synthesizing extracts incubated with purified preparations of colicin E3 showed a marked decrease of their protein synthesizing activity. Incubation of ribosomes prepared from S-30 extracts with E3 resulted in their inactivation. Their 16S RNA was found to lose a terminal fragment, similar in size to the fragment released uponin vivo treatment with E3 . Extracts derived from colicinogenic strains are resistant to the action of E3 in vitro .