Open AccessMultimodal on-chip nanoscopy and quantitative phase imaging reveals the nanoscale morphology of liver sinusoidal endothelial cells
Author(s) -
Ankit Butola,
David A. Coucheron,
Karolina Szafranska,
Azeem Ahmad,
Hong Mao,
Jean-Claude Tinguely,
Peter McCourt,
P. Senthilkumaran,
Dalip Singh Mehta,
Krishna Agarwal,
Balpreet Singh Ahluwalia
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2115323118
Subject(s) - materials science , nanoscopic scale , microscopy , interference microscopy , microscope , optical microscope , optics , scanning electron microscope , nanotechnology , physics , composite material
Significance We propose the integration of chip-based optical nanoscopy with high spatially sensitive quantitative phase microscopy to obtain three-dimensional (3D) morphology of liver sinusoidal endothelial cells (LSEC). LSEC contain large numbers of transcellular nanopores —“fenestrations”—in the plasma membrane, typically clustered in groups of 10 to 50 within areas called sieve plates. Determining the diameter and the height of fenestrated regions is an important indicator of a cell’s functionality, and these dimensions can be influenced by agents such as drugs. Our proposed multimodal microscope offers a solution for 3D nanoscale characterization of fenestration diameter and measurement of the optical thickness of the sieve plates.