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Significance Glaucoma is a leading cause of irreversible blindness worldwide. A major risk factor is increased intraocular pressure (IOP), and all current treatment strategies for slowing disease progression center on IOP reduction. Increased IOP generally results from reduced drainage of aqueous humor through two routes in the anterior segment of the eye called the conventional and uveoscleral outflow pathways. We use high-throughput single-cell RNA sequencing to identify cell types within these pathways and the genes that they express both in humans and in four model species frequently used to study IOP regulation and glaucoma.

Cell atlas of aqueous humor outflow pathways in eyes of humans and four model species provides insight into glaucoma pathogenesis