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Significance Binding of parathyroid hormone (PTH) to its cognate receptor (PTHR) has been shown to induce prolonged cAMP responses from early endosomes. While the receptor also couples to Gq/11 , whether this pathway modulates sustained cAMP remains unexplored. Here, we show that lack of Gq/11 activation reduces the duration of PTH-induced cAMP generation. Our findings reveal that this effect occurs at the level of the G protein, rather than downstream effectors, via provision of Gβγ subunits at the cell surface. We demonstrate that Gq/11 -derived Gβγ subunits act in a phosphoinositide-3 kinase-dependent manner to promote assembly of ternary PTHR–βarrestin–Gβγ complexes that are required for PTH-mediated sustained cAMP from endosomes. These findings provide insights into the spatiotemporal regulation of GPCR signaling.

G q/11 -dependent regulation of endosomal cAMP generation by parathyroid hormone class B GPCR

G _q/11 -dependent regulation of endosomal cAMP generation by parathyroid hormone class B GPCR