English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Significance Growth of NIH 3T3 cells in an optimized medium produced transformed foci after reaching confluence in the absence of treatment with carcinogens or transfection with tumor DNA. Dynamic experiments showed the first response of the crowded cells was gradual population-wide increases in saturation density followed by transformed foci. The latter produced malignant tumors when injected into mice. The field of cells around the foci became increasingly susceptible to transformation. Analysis of the data revealed that transformation was driven by epigenetic selection of cell populations that grew well at high densities rather than genetic instability. That conclusion is reinforced by two other methods of selection, one at low cell density in low serum concentration and the other by suspension in agar.

Dynamics of cell transformation in culture and its significance for tumor development in animals