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Elasticity is a fundamental cellular property that is related to the anatomy,functionality and pathological state of cells and tissues. However, currenttechniques based on cell deformation, atomic force microscopy or Brillouinscattering are rather slow and do not always accurately represent cellelasticity. Here, we have developed an alternative technique by applying shearwave elastography to the micrometer scale. Elastic waves were mechanicallyinduced in live mammalian oocytes using a vibrating micropipette. These audiblefrequency waves were observed optically at 200,000 frames per second andtracked with an optical flow algorithm. Whole cell elasticity was then mappedusing an elastography method inspired by the seismology field. Using thisapproach, we show that the elasticity of mouse oocyte is decreased when theoocyte cytoskeleton is disrupted with cytochalasin B. The technique is fast(less than 1 ms for data acquisition), precise (spatial resolution of a fewmicrometers), able to map internal cell structures, robust, and thus representsa tractable novel option for interrogating biomechanical properties of diversecell types.

Ultrafast imaging of cell elasticity with optical microelastography