English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Significance Fluorescent proteins (FPs) started their incredible, colorful journey in bioimaging and biomedicine with the extraction and purification of GFP from the Pacific jellyfishAequorea victoria more than 50 years ago. Recently, an expanded palette of genetically encodable Ca2+ -sensing FPs have paved the way to image neural activities and important biological processes where Ca2+ is the ubiquitous messenger. To unravel the molecular choreography of FPs engineered for visualizing Ca2+ movement, we study the embedded chromophore upon photoexcitation and monitor its subsequent excited-state structural evolution with femtosecond Raman spectroscopy. The vivid insights on H-bonding network and functional roles played by strategic mutations provide a deep understanding of excited-state processes in biology and will guide future bioengineering efforts toward better biosensors.

Excited-state structural dynamics of a dual-emission calmodulin-green fluorescent protein sensor for calcium ion imaging