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We initiated our study (1) following a publication by Nishioka et al. (2), which reported similar levels of TEAD4 expression in both inner cell mass (ICM) and trophectoderm (TE) nuclei (figure 2c of ref. 2). Thus, we tested multiple mouse blastocysts (n = 35) assuming TEAD4 will be nuclear in the ICM. However, as shown in the study (1), we detected TEAD4 only within the cytoplasm of ICM lineage cells. As indicated in the study (1), specific knockdown of TEAD4 in mouse trophoblast cells validated the specificity of the TEAD4 antibody (ab58310; Abcam). Hirate et al. (3), in their letter, also validated the specificity of the TEAD4 antibody. Moreover, in contrast to the initial Nishioka et al. study (2) and in agreement with our study (1), they confirmed that TEAD4 expression decreases in the ICM lineage …

Reply to Sasaki et al.: TEAD4 is predominantly cytoplasmic in the inner cell mass of mouse blastocysts