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Frerichs et al. (1) view the prevalence of Vibrio cholerae non-O1/O139 skeptically in the early weeks of the Haitian epidemic (2). Unexpected findings spawn skeptics. Setting aside differences in sampling time frames, differences in recoveries might readily be traced to comprehensive sampling and methodologies. Using a combination of thiosulphate-citrate-bile salt-sucrose and taurocholate tellurite gelatin agar medium to substantiate detection of both sucrose fermenting and nonfermenting V. cholerae reported in this region (3), we picked multiple single-colony isolates (average of 4, depending on availability of well-separated colonies and colony morphologies) on each original plate, which were screened systematically, using a battery of specific biochemical, serological, and molecular methods. Sensitive and discriminating, but more expensive and labor- and time-intensive, our …

Reply to Frerichs et al.: Chasing the genetic diversity, not source attribution