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Our newly published study of the proton translocation efficiency of cytochrome cbb3 (1), on the basis of which we questioned the recent results by Han et al. (2), has inspired the latter authors to redetermine the proton-pumping stoichiometry of the Rhodobacter capsulatus enzyme in cells at a higher pH to avoid an artifact brought about by proton release from N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) on oxidation (3). Despite the improved experimental conditions, …

Reply to Murali et al.: Proton translocation stoichiometry of cbb 3 -type cytochrome c oxidase

Reply to Murali et al.: Proton translocation stoichiometry of cbb ₃ -type cytochrome c oxidase