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We are pleased that the results of Kohn (1) so strongly support our findings published earlier in PNAS (2). Both studies (1, 2) analyzed published measurements of the carbon isotope composition of plants and quantified relationships between isotopic fractionation and environmental factors at large spatial scales. These relationships will be useful when interpreting archives of plant-derived carbon, such as fossils, soil organic matter, and plant biomarkers. Kohn (1) emphasized differences between the two papers, particularly the coefficients and proportion of variance explained in regression models, but the major findings were the same: water availability [estimated by mean annual precipitation (MAP)] explains most of the observed variability in δ13C values …

Clarifying the influence of water availability and plant types on carbon isotope discrimination by C3 plants