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To determine easily and with precision the inhibitors of fibrinolysis, we have developed a method studying the critical Urokinase amount that can produce the fibrin plate lysis in 20 hours of incubation (37° C), by its addition to the fibrinogen solution when the fibrin plate is prepared. Placed on the coagulated fibrin layer samples (20 μl) of blood, plasma, serum or isolated inhibitors from euglobulins supernatant, we obtained circular areas of unlysed fibrin; plotting sample dilution, in logarithmic scale, against unlysed fibrin diameter, in decimal scale, a linear response is obtained. To give a standarization pattern we assayed a Trasylol curve that showed a linear response between 1 and 40 I.U./ml., so the results can be expressed in diameters of unlysed fibrin or in Trasylol units. As standard can also be used Iniprol in a range of 0.5 to 8 I.U./ml. or a pool of normal plasma, or whatever we have to evaluate in progressive dilutions. We have been contrasted this method with thromboelastographic and Blix ones, obtaining correlative results.  This is an easy performance method that can be of clinical interest to study a great number of samples and to control the fibrinolytic agent treatment.

Method for the Control of Fibrinolytic Inhibitors