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The platelet factor 3 immuno-injury technic (Karpatkin and Siskind, Blood 33:795,1969) has been evaluated as a test for the detection of anti-platelet antibodies in 126 patients with quantitative and qualitative platelet disorders. Plasmatic acid phosphatases in platelet-poor plasma (PAP-PPP) were simultaneously determined in a selected group of patients. Also the effect of serum incubation (from patients and controls) on acid phosphatase availability of platelet-rich plasma (APA-PRP) was investigated.  Anti-platelet antibodies were present in 52% of patients fulfilling the diagnostic criteria for idiopathic thrombocytopenic purpura and in 38% of patients with miscellaneous conditions. The following table express the results of acid phosphatase determina studied:  Platelet destruction is the probable cause of the thrombocytopenia in the group with negative anti-platelet antibodies. Further, their serum lacks membrane-injury action. On the other hand the results in the group with positive antibodies suggest that heterogeneous immunologic mechanisms are operative

Studies on Anti-Platelet Antibodies. Its Correlation with ACID Phosphatases. Preliminary Results