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Whether the three measurable parameters of factor VIII (procoagulant activity, VIII:C; ristocetin cofactor activity, VIIIR:WF; and factor VIII related antigen, VIIIR:AG) reside on a single protein remains disputed. A solid phase immunoadsorption system, in which homologous antibodies to VIII:C arising in haemophiliacs were insolubilized onto Sepharose, was used to examine the action of such antibodies and the inter-relationship between VIII:C, VIIIR:WF and VIIIR:AG. Homologous antibodies were shown to bind specifically VIII:C and to induce a spontaneous separation of VIII:C from VIIIR:WF/VIIIR:AG. The bond between VIII:C and the homologous antibodies bound to Sepharose appeared to be very stable and could not be broken with the usual antigen-antibody dissociating agents. Following prolonged incubation with antibody-sepharose, concentrated VIIIR:WF/VIIIR:AG (20 u/ml), completely devoid of VIII:C and inhibitor-neutralizing activity, was obtained. The loss of VIII:C had no detectable effect on the molecular size, antigenicity or electrophoretic mobility of the original molecule. The concentrated VIIIR:WF/VIIIR:AG was used to absorb heterologous antisera raised against factor VIII. Specific heterologous antisera to VIII:C, no longer neutralizing VIIIR:WF nor precipitating with VIIIR:AG, were obtained. Immunization of rabbits with VIIIR:WF/VIIIR:AG resulted in antisera which potently neutralized VIIIR:WF and precipitated with VIIIR:AG but also weakly neutralized VIII:C. These antibodies, like 4 other heterologous antibodies to Factor VIII studied, did not neutralize VIII:C which had been dissociated from VIIIR:WF/VIIIR:AG.  The results indicate that VIII:C and VIIIR:WF/VIIIR:AG are two different, but linked entities.

Immunological Evidence that Human Factor VIII is Composed of Two Linked Moieties