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To investigate the effect of glucocorticoid on adipocytes metabolism and miRNAs that may be involved in adipocyte differentiation, primary porcine preadipocytes were treated with 10-6 M dexamethasone and RU486 (a glucocorticoid receptor antagonist) for 48 h. PPAR-γ (peroxisome proliferators-activated receptor-γ), and C/EBP-β (CCTTA enhancer binding protein-β) gene and protein expression were measured. The expressions of miRNAs predicted to directly target C/EBP-β were determined, and the functions of the potential miRNAs were verified. The results showed that the triglyceride content in cultured adipocytes increased significantly after 10-6 M dexamethasone treatment for 48 h, whereas the cell viability did not differ among the four groups (

miRNA-374 Regulates Dexamethasone-induced Differentiation of Primary Cultures of Porcine Adipocytes