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This study evaluated the  in vitro  anthelmintic activity of a liquorice ( Glycyrrhiza glabra ) root aqueous extract and of glycyrrhetinic acid at 30, 10, 5, 1, and 0.5 mg/mL against sheep gastrointestinal nematodes (GINs), using the egg hatch test (EHT), the larval development test (LDT), and the larval migration inhibition test (LMIT). The compounds were applied on a mixture of GIN eggs and larvae, mainly  Trichostrongylus  spp. and  Teladorsagia/Ostertagia  spp. Cytotoxicity assays were also performed. In the EHT, both candidates showed significant concentration-dependent efficacy and were significantly more effective ( p    2.0-fold lower when compared to  G. glabra  (12.25 mg/mL). Analysis and previous findings indicated low toxicity for both compounds. The results obtained encourage  in vivo  studies aimed at evaluating the potential use of the tested compounds as natural de-wormers in ruminants.

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<i>In vitro</i> anthelmintic activity of an aqueous extract of <i>Glycyrrhiza glabra</i> and of glycyrrhetinic acid against gastrointestinal nematodes of small ruminants

In vitro anthelmintic activity of an aqueous extract of Glycyrrhiza glabra and of glycyrrhetinic acid against gastrointestinal nematodes of small ruminants