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Acld-resistant mutants of Lactococcus lactis. In ail environments, bacteria are submitted to stress conditions which compromise cell growth and survival. It is now established that bacteria have inducible stress resistance mechanisms which favor bacterial survival under dif- ficult conditions. These resistance mechanisms may be useful for improving strain survival in tech- nological processes. Using L. lactis, we selected mutants more resistant to acid stress than the wild- type strain, MG 1363. Results of genetic and phenotypic characterization of these mutants established that strain survival is improved compared to MG 1363 in a single or up to three dif- ferent stress conditions, and sometimes by a factor of 2 000. This work shows that in L. lactis,stress responses can overlap and that a mutated strain can be more appropriate than the wild-type to sur- vive abrupt environmental changes. Such strains may have improved properties in industrial processes. © Inra/Elsevier, Paris. acid stress 1Lactococcus lactis 1 acid-resistance 1 multi-stress resistance 1 transposition

Mutants de Lactococcus lactis résistants à l'acidité