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Controlled pollination techniques were developed in grafted material in 3 clones (27 trees). A total of 2327 flowers were pollinated. At anthesis, the operculum was removed manually and emasculation was done with a blade. The flowers were then isolated with unwoven terylene bags. Optimum stigma receptivity, shown by an exudate, was determined using controlled pollinations made over 2 d intervals, from d 4 to d 14, in groups of 60 flowers. In each group, the proportion of flowers with stigmatic exudations was recorded. Controlled pollinations were made with a brush.

Hybridization techniques and frost tolerance studies in intraspecific hybrids of Eucalyptus globulus Labill