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The studies present the results of morphological, cultural and genetic analysis of the ITS1-ITS4 region of the autochthonous yeast strains genome by using the HaeIII restriction enzyme. On the red and white grapes varieties, based on the morphology of the cells, autochthonous strains belonging to the genus  Saccharomyces  prevail – 83.3%, what is confirmed by genetic analysis of rDNA ITS region. Restriction analysis showed that all strains of the genus  Saccharomyces  belong to the species  Saccharomyces cerevisiae / S. paradoxus . The percentage of  Saccharomyces  isolated on the Pervenets Magaracha variety is 86.7%, Krasnostop Anapsky - 80%. The  non-Saccharomyces  yeast had a product size of 750 bp, presumably of the species  Hanseniaspora uvarum .

PCR-RFLP ITS-based analysis of wine yeast autochthonous strains isolated from different grape cultivars in Taman subregion