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The article provides a method of isolating a lipase producer and identification methods by classical methods in microbiology of an isolated micromycete producing lipase. The species affiliation of  Penicillium hordei  has been determined. The temperature of micromycete cultivation was studied, as well as the dynamics of  Penicillium hordei  lipase biosynthesis. Screening plans were also carried out (Plackett-Berman Plan), then optimization according to the steep climb plan and an experiment based on the plan of a full-factor experiment (three-level CFE) was also conducted to determine the optimal concentrations that are significant for lipase biosynthesis of components. A mathematical model is constructed that describes the relationship between the influence of two significant factors on the volumetric activity of  P. Hordei  culture fluid. The most optimal composition for the culture fluid for lipase biosynthesis,  Penicillium hordei  micromycete, was compiled.

Biosynthesis lipase from the fungusPenicillium hordeiisolated from waste fat