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The conditions to extract alchol soluble proteins of leaves of  Wedelia trilobata (L.) Hitchc.  were discussed. When the extractant was alcohol, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio was 1:10, temperature for extraction was 50°Cand the extraction period was 2hrs, the concentration of alcohol solution needed for the best extraction effect was 95% (V/V). When the extractant was 75% alcohol solution, the temperature for extraction was 50°Cand the extraction period was 2hrs, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio needed for the best extraction effect was 1:20. When the extractant was 75% alcohol solution, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio was 1:10 and the extraction period was 2 hrs, the temperature needed for the best extraction effect was 55°C. When the extractant was 75% alcohol solution, the weight of leave(g) -to- volume of 75% alcohol solution (mL) ratio was 1:10 and the temperature for extraction was 50°C, the extraction period needed for the best extraction effect was 2hrs.

Preliminary researches on conditions to extract alchol soluble proteins of leaves of Wedelia trilobata (L.) Hitchc.