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Upgrading of low biodegradable waste activated sludge (WAS) accomplished through supplement the hydrolysis step with natural enzymes source. Whereas, WAS is rich in particulate fractions in terms of total chemical oxygen demand (tCOD), total suspended solids (TSS) and volatile suspended solids (VSS) of 15.78, 14.92 and 12.15 g/L, respectively. Therefore, carica papaya enzymes were utilized to break down the peptide ponds in protein molecules such as papain and protease, as well as, lipases that catalyzed the degradation of lipids. The optimum mixture between papain, protease, and lipase enzymes was found to be 3: 1: 2 while the optimum enzyme concentration was 8%. This conditions was attributed to enhance the H2 productivity form WAS by 97.8%

Enrich waste activated sludge digestibility via natural enzyme supplementation