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Changes in chemical composition of barley straw following the treatment were seen as an increase in the nitrogen content of the cell-wall fraction and as a partial loss of ester-linked phenolic acids and acetyl groups. Ammonia treatment promoted access by rumen microorganisms to the luminal surface of parenchyma and sclerenchyma cells apparently due to an alteration in the fragility of a thinner, rigid layer covering the inner surface of cell walls. Two measures of the X-ray diffraction pattern in treated straw indicated that treatment of barley straws produced a significant reduction in crystallinity index values of 14 24 %. The water retention of the treated barley straw was 30 % greater than that of the untreated straw following centrifugation at 300 x g and 3000 x g. A slightly higher swollen volume was also observed for water-saturated ammonia-treated barley straw, although the difference was not significant.

Ammoniation of barley straw : effect on anatomical and physicochemical characteristics of the cell walls