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Total RNA and poly (A+) RNA was isolated from both rat and cow duodenal scrapings by the guanidinium-cesium chloride method and oligo d(T) cellulose chromatography as described by Glimm et al (1992, J Dairy Sci, 75, 2687-2705). A rat CCK cDNA (JE Dixon, Purdue University, West Lafayette, IN, USA) was subcloned into pBluescript® II SK (Stratagene). A radiolabelled (32 P-CTP) CCK antisense RNA probe was generated using T3 polymerase (Promega). The CCK probe was used in a Northern hybridization assay to detect CCK mRNA in total and poly (A+) samples. RNA depleted of polyadenylated RNA (poly (A-)) served as negative control. Autoradiographic signal were analyzed using

Cholecystokinin gene is expressed in bovine duodenal tissue