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Three lactating goats (body weight 39.4-45.5 kg; milk yield 2.0!.6 kg/d) were used during midlactation. They were fed a concentrate containing mainly maize (Champredon et al, 1990) and a mixture of meadow and lucerne chopped hays (2:1) both given at variable levels. Dry matter intake varied from 1 430 and 2 630 g/d and the PDI (digestible protein in the intestine; Jarrige, 1989) intake from 130 to 280 g/d. The goats were surgically fitted with catheters in the carotid artery, the portal vein and a mesenteric vein. Portal plasma flow was measured by continuous infusion of para-aminohippuric acid via the mesenteric catheter. The amino-acid appearance rate from the portal-drained viscera was obtained from portal plasma flow, and the differences between venous and carotid plasma concentrations. Three to 4 measurements (corresponding to different levels of dietary intake) were performed on each goat on different days. The portal plasma flow of the lactating goats used in the present study ranged from 58 to 69 ml/min/kg body weight. The coefficients of the linear regression relating the net portal appearance rate of amino acids (NPA in g/d) to the PDIintake (g/d) are given in table I. All regression coefficients (b) were significant (P < 0.01 ). Intercepts (a) were significantly different from the origin (P < 0.05) except for threonine (P = 0.29).

Relationship between protein intake (PDI) and net portal appearance rate of amino acids in lactating goats