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The N degradability of soya-bean meal (SBM), meat and bone meal (MBM) and wheat bran (WB) was measured using the nylon bag procedure (0rskov and McDonald, 1979) in 2 adult Friesian steers receiving twice daily a diet based or concentrate and meadow hay (50:50, 13.5% CP, 10 kg DM/d). Two d before starting and during the incubation period (!SNH4)2S04 (99 atom% excess, 3 g/d) was infused continuously through the rumen cannula. Rumen bacterial N in the residues (as % of total residual N) was calculated from the ratio: (15 N atom % excess in the residue/!SN atom % excess in the bacteria) x 100. Whole rumen content was sampled in each steer 0, 3, 6 and 9 h after feeding. Liquid and solid associated bacteria (LAB and SAB) were extracted as described by LegayCarmier and Bauchart (1989). N was determined by the Kjeldahl method. Isotope-ratio analyses were performed according to the Kjeldahl-Rittenberg method using a doublecollector mass spectrometer (VG, SIRA 12).

The use of 15N for the determination of in sacco bacterial contamination of 3 concentrate feeds and N degradability of feeds in the rumen

The use of ¹⁵N for the determination of in sacco bacterial contamination of 3 concentrate feeds and N degradability of feeds in the rumen