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The induced proliferative response of the human derived neuroblastoma cell (IMR32), with the cell proliferation medi- ating factor, is much milder compared to other cell strains, and develops cellular clusters which are characterized by numerous extensions of dendrites. Presuming this phenomenon to be one of the induced neuronal differentiation due to genetic alterations , we preliminary studied to elucidate the changes in gene expression of the IMR32 by mRNA differential display analysis, moni- toring polymerase chain reaction (PCR) products by non-gel molecular sieving capillary electrophoresis in linear polyacrylamide solution. The CE fingerprints revealed a number of peaks with differential expression patterns.

analusis

Differential display analysis of gene expression accompanied by neurite outgrowth of human neuroblastoma Cell IMR32 using non-gel molecular sieving capillary electrophoresis