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Single stage separation systems have limitations in resolving multicomponent mixtures and two-dimensional systems have higher peak capacities. A valveless interface for hyphenating Reversed-phase Microchromatography ( μ-RPLC) and Capillary Zone Electrophoresis (CZE) -highly orthogonal separation methods- is presented, which main innovation is a third electrode. Design and operation of the interface as well as some preliminary results obtained by combining FIA and μ-RPLC with CZE are shown.

Valveless interface for two-dimensional reversed-phase microcromatography (μ-RPLC)-capillary zone electrophoresis (CZE)