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The 2-methoxyethyl 1-methylethyl 2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate was obtained in a 99.8% pure form from raw material by preparative-scale high performance liquid chromatography. This compound is an impurity in the industrial process of nimodipine. The chromatographic conditions were optimized to achieve any efficient purification in the sh ort- est time. A reversed-phase preparative-scale HPLC column was used. This work demonstrates how gram quantities of pure mate- rial can be conveniently obtained using preparative HPLC, even when the components in a mixture are difficult to resolve.

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Purification of 2-methoxyethyl 1-methylethyl 2, 6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate by preparative high-performance liquid chromatography