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THE colorimetric method, because of its simplicity and the rapidity with which determinations may be made, has been the object of many biochemical investigations. Levy, Rowntree and Marriott [1915] devised the dialysis method in which phenol red was added to the dialysate of the blood, and the resulting colour compared to a set of standards. Later, Dale and Evans [1920-21] improved this method by preventing the 1088 of C02 from the system. Cullen [1922] eliminated the error due to the Donnan effect in the dialysis method by a procedure employing diluted plasma, in which an empirical correction, determined by a comparison of the colorimetric results obtained at 200 with those obtained by the hydrogen electrode at 380, allowed for protein, salt, dilution, and temperature effects. Employing the Gillespie principle, Hastings and Sendroy [1924] developed a system of bicolour standards which permitted the colorimetric determination of the pH of plasma or of whole blood at 380, obviating the use of the empirical Cullen C correction.

A comparison of the colorimetric and electrometric methods for the determination of the pH of serum or plasma