English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

A novel, selective and sensitive switch-on fluorescent sensor for Hg(2+) and switch-off fluorescent probe for biothiols was developed by using [Ru(bpy)₂(pip)](2+) as the signal reporter and graphene oxide (GO) as the quencher. Due to the affinity of GO towards single-stranded DNA (ss-DNA) and [Ru(bpy)₂(pip)](2+), the three components assembled, resulting in fluorescence quenching. Upon addition of Hg(2+), a double-stranded DNA (ds-DNA) via T-Hg(2+)-T base pairs was formed, and [Ru(bpy)₂(pip)](2+) intercalated into the newly formed ds-DNA. Then, [Ru(bpy)₂(pip)](2+) and ds-DNA were removed from the surface of GO, resulting in the restoration of fluorescence. Subsequently, upon addition of biothiols, Hg(2+) was released from ds-DNA, due to the higher affinity of Hg(2+) to the sulfur atoms of biothiols, which could induce ds-DNA unwinding to form ss-DNA. Then ss-DNA and [Ru(bpy)₂(pip)](2+) were adsorbed on the surface of GO, the fluorescence of [Ru(bpy)₂(pip)](2+) was quenched again. Therefore, the changes in emission intensity of [Ru(bpy)₂(pip)](2+) directly correlated to the amount of detection target (Hg(2+) or biothiols) in solution. The assay exhibited high sensitivity and selectivity, with the limits of detection for Hg(2+), cysteine (Cys) and glutathione (GSH) to be 2.34 nM, 6.20 nM and 4.60 nM, respectively.

A label-free fluorescent probe for Hg2+ and biothiols based on graphene oxide and Ru-complex