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The human orthologue of the tumor suppressor protein FBW7 is encoded by the Drosophila  archipelago (ago)  gene. Ago is an F-box protein that gives substrate specificity to its SCF ubiquitin ligase complex. It has a central role in multiple biological processes in a tissue-specific manner such as cell proliferation, cellular differentiation, hypoxia-induced gene expression. Here we present a previously unknown tissue-specific role of Ago in spermatid differentiation. We identified a classical mutant of  ago  which is semi-lethal and male-sterile. During the characterization of  ago  function in testis, we found that  ago  plays role in spermatid development, following meiosis. We confirmed spermatogenesis defects by silencing  ago  by RNAi in testes. The  ago  mutants show multiple abnormalities in elongating and elongated spermatids, including aberration of the cyst morphology, malformed mitochondrial structures, and individualization defects. Additionally, we determined the subcellular localization of Ago protein with mCherry-Ago transgene in spermatids. Our findings highlight the potential roles of Ago in different cellular processes of spermatogenesis, like spermatid individualization, and regulation of mitochondrial morphology.

The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis