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The desert pioneer plant  Stipagrostis pennata  plays an important role in sand fixation, wind prevention, and desert ecosystem recovery. An absence of reference genes greatly limits investigations into the regulatory mechanism by which  S. pennata  adapts to adverse desert environments at the molecular and genetic levels. In this study, eight candidate reference genes were identified from rhizosheath development transcriptome data from  S. pennata , and their expression stability in the rhizosheaths at different development stages, in a variety of plant tissues, and under drought stress was evaluated using four procedures, including geNorm, NormFinder, BestKeeper, and RefFinder. The results showed that  GAPDH  and  elF  were the most stable reference genes under drought stress and in rhizosheath development, and  ARP6  and  ALDH  were relatively stable in all plant tissues. In addition,  elF  was the most suitable reference gene for all treatments. Analysis of the consistency between the reverse transcription-quantitative PCR (RT-qPCR) and RNA sequencing data showed that the identified  elF  and  GAPDH  reference genes were stable during rhizosheath development. These results provide reliable reference genes for assuring the accuracy of RT-qPCR and offer a foundation for further investigations into the genetic responses of  S. pennata  to abiotic stress.

Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata