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Acinetobacter baumannii  is a frequent cause of the nosocomial infections. Herein, a novel isothermal amplification technique, multiple cross displacement amplification (MCDA) is employed for detecting all  A. baumannii  strains and identifying the strains harboring  bla   OXA-23-like   gene. The duplex MCDA assay, which targets the  pgaD  and  bla   OXA-23-like   genes, could identify the  A. baumannii  isolates and differentiate these isolates harboring  bla   OXA-23-like   gene. The disposable lateral flow biosensors (LFB) were used for analyzing the MCDA products. A total of sixty-eight isolates, include fifty-three  A. baumannii  strains and fifteen non- A. baumannii  strains, were employed to optimize MCDA methods and determine the sensitivity, specificity and feasibility. The optimal reaction condition is found to be 63 °C within 1 h, with limit of detection at 100 fg templates per tube for  pgaD  and  bla   OXA-23-like   genes in pure cultures. The specificity of this assay is 100%. Moreover, the practical application of the duplex MCDA-LFB assay was evaluated using clinical samples, and the results obtained from duplex MCDA-LFB method were consistent with conventional culture-based technique. In sum, the duplex MCDA-LFB assay appears to be a reliable, rapid and specific technique to detect all  A. baumannii  strains and identify these strains harboring  bla   OXA-23-like   gene for appropriate antibiotic therapy.

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Identification of Acinetobacter baumannii and its carbapenem-resistant gene blaOXA-23-like by multiple cross displacement amplification combined with lateral flow biosensor