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A total amount of 116 fungal strains, belonging to 30 genera, were acquired from the rhizosphere soil and plant of  Galinsoga parviflora . A strain SYPF 7336, isolated from the rhizospheric soil, was identified as  Seltsamia galinsogisoli  sp. nov., by morphological and molecular analyses, which displayed high antibacterial activity. In order to study the secondary metabolites of  Seltsamia galinsogisoli  sp. nov., nine compounds were successfully seperated from the strain fermentation broth, including two new compounds and seven known compounds. Their structures were elucidated based on spectral analysis including 1D and 2D NMR. All the seperated compounds were evaluated for their antimicrobial activities. Compounds  2, 5  and  1  displayed antimicrobial activities against  Staphylococcus aureus  with MIC values of 25, 32 and 75 μg/mL, respectively. Moreover, morphological observation showed the coccoid cells of  S. aureus  to be swollen to a volume of 1.4 to 1.7-fold after treatment with compounds  1 ,  2  and  5 , respectively. Molecular docking was carried out to investigate interactions of filamentous temperature-sensitive protein Z (FtsZ) with compounds  1 ,  2  and  5 .

New antimicrobial compounds produced by Seltsamia galinsogisoli sp. nov., isolated from Galinsoga parviflora as potential inhibitors of FtsZ