English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Tomato wilt pathogen  Fusarium oxysporum  f. sp.  lycopersici  ( Fol ) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of  Fol  races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in  secreted in xylem 3  ( SIX3 ) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in  SIX3  of  Fol . Loop-mediated isothermal amplification (LAMP) of  SIX3  gene with the universal QProbe as well as two joint DNAs followed by annealing curve analysis allowed us to specifically detect  Fol  and discriminate race 3 among other races in about one hour. Our developed method is applicable for detection of races of other plant pathogenic fungi as well as their pesticide-resistant mutants that arise through point mutations in a particular gene.

Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi