English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We showed recently that the live-attenuated Δ lpp  Δ msbB  Δ ail  and Δ lpp  Δ msbB::ailL2  mutants of  Yersinia pestis  CO92 provided short-term protection to mice against developing subsequent lethal pneumonic plague. These mutants were either deleted for genes encoding Braun lipoprotein (Lpp), an acetyltransferase (MsbB) and the attachment invasion locus (Ail) (Δ lpp  Δ msbB  Δ ail ) or contained a modified version of the  ail  gene with diminished virulence (Δ lpp  Δ msbB::ailL2 ). Here, long-term immune responses were first examined after intramuscular immunisation of mice with the above-mentioned mutants, as well as the newly constructed Δ lpp  Δ msbB  Δ pla  mutant, deleted for the plasminogen-activator protease ( pla ) gene instead of  ail .  Y. pestis- specific IgG levels peaked between day 35 and 56 in the mutant - immunised mice and were sustained until the last tested day 112. Splenic memory B cells peaked earlier (day 42) before declining in the Δ lpp  Δ msbB::ailL2  mutant-immunised mice while being sustained for 63 days in the Δ lpp  Δ msbB  Δ ail  and Δ lpp  Δ msbB  Δ pla  mutant-immunised mice. Splenic CD4 +  T cells increased in all immunised mice by day 42 with differential cytokine production among the immunised groups. On day 120, immunised mice were exposed intranasally to wild-type (WT) CO92, and 80–100% survived pneumonic challenge. Mice immunised with the above-mentioned three mutants had increased innate as well as CD4 +  responses immediately after WT CO92 exposure, and coupled with sustained antibody production, indicated the role of both arms of the immune response in protection. Likewise, rats vaccinated with either Δ lpp  Δ msbB  Δ ail  or the Δ lpp  Δ msbB  Δ pla  mutant also developed long-term humoral and cell-mediated immune responses to provide 100% protection against developing pneumonic plague. On the basis of the attenuated phenotype, the Δ lpp  Δ msbB  Δ ail  mutant was recently excluded from the Centers for Disease Control and Prevention select agent list.

npj vaccines

Immunisation of two rodent species with new live-attenuated mutants of Yersinia pestis CO92 induces protective long-term humoral- and cell-mediated immunity against pneumonic plague