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One hundred and nine strains of Haemophilus influenzae type b were subtyped by sodium dodecyl sulphate polyacrylamide gel electrophoresis of whole-cell polypeptides. Twenty-one strains from England, 44 from Scotland, 8 from Sweden, 6 from the Netherlands and 30 from the USA were examined. Some of these strains had been subtyped by outer membrane protein analysis; most of the strains had been isolated from cases of invasive disease. Comparison of polypeptide profiles using the Dice coefficient of similarity showed that the majority of European strains were closely related and formed a single large group. Four smaller groups were identified; three of these included American and European strains, indicating a world-wide distribution of subtypes. However, the common European and American subtypes fell into different groups, indicating the existence of marked geographical variations in subtype frequency.

epidemiology and infection

Subtyping of<i>Haemophilus influenzae</i>type b strains from Europe and North America by SDS—PAGE of whole-cell polypeptides: the geographical distribution of subtypes

Subtyping ofHaemophilus influenzaetype b strains from Europe and North America by SDS—PAGE of whole-cell polypeptides: the geographical distribution of subtypes