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The rat and mouse cubes described in the first paper of this series have now been in continuous use in this laboratory for over 4 years, and give excellent results for growth and maintenance. It was noted, however, from the start, that they were quite unsuitable for breeding animals (Parkes, 1946). First litter production was seriously impaired even in mice which had been fed on the cubes only during the experiment. By contrast, the breeding diet then in use, a freshly prepared meal mixed as required, which contained 27 % full-cream dried milk, gave very good results. This diet, however, was both expensive and wasteful, and work was undertaken to determine the reasons for the failure of the maintenance cubes to support reproduction. The composition of the two diets is as follows:

The feeding and breeding of laboratory animals: VII. Methods of testing the adequacy of diets for breeding mice