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A technique is presented for the titration of the virus of foot-and-mouth disease in culture in surviving epithelial tissue from the tongues of cattle. The cultures are incubated in cups on Perspex plates, and the detection of virus multiplication is by a complement-fixation test made on the culture in each cup. On the basis of comparative titrations in culture and in cattle, the method has been found to be as sensitive for the detection of virus as the titration by intradermal inoculation of the tongue of cattle. The method can also be applied in the detection of antibody in neutralization tests. We wish to record our thanks to Messrs E. Scoates and P. Mitchell for their technical assistance, and to Messrs H. M. Smith, R. H. Compton and R. L. Jackson for their part in the design and fabrication of various bottle rotators, plate shakers, Perspex lids and the inoculating box.

Titration of the virus of foot-and-mouth disease in culture