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CONTENTS. PAGE I n t r o d u c t i o n . . . . . . . . . . . . . . . 1 7 2 T e c h n i q u e 1 7 4 P r e l i m i n a r y E x p e r i m e n t s w i t h P e p t o n e S o l u t i o n s u b s t i t u t e d f o r A n t i g e n i n t h e C o m p l e m e n t f i x a t i o n T e s t w i t h N o r m a l S e r u m . . . . . . . . . 1 7 5 C o m p a r i s o n of S e r a of D i f f e r e n t S p e c i e s 1 7 7 T h e I n f l u e n c e of T e m p e r a t u r e . . . . . . . . . . . . 1 8 1 T h e A b s e n c e of R e a c t i n g P r o p e r t i e s f r o m t h e S e r u m of Y o u n g A n i m a l s . . . . 1 8 1 A b s o r p t i o n E x p e r i m e n t s . . . . . . . . . . . . . 1 8 3 F u r t h e r E x p e r i m e n t s w i t h P e p t o n e S o l u t i o n . . . . . . . . . 1 8 3 S u b s t a n c e s C a p a b l e o f P r o d u c i n g C o m p l e m e n t f i x a t i o n w i t h N o r m a l S e r u m . . . 1 8 4 E f f e c t of C h o l e s t e r o l i s a t i o n . . . . . . . . . . . . 1 8 8 C o m p l e m e n t f i x a t i o n E f f e c t s o b t a i n e d w i t h W a s s e r m a n n p o s i t i v e a n d n e g a t i v e H u m a n S e r a a l o n g w i t h v a r i o u s " P s e u d o a n t i g e n s " . . . . . . . . 1 8 9 T h e r m o l a b i l i t y o f S e r u m P r o p e r t i e s 1 9 0 D i s c u s s i o n . . . . . . . . . . . . . . . 1 9 3 S u m m a r y a n d C o n c l u s i o n s . . . . . . . . . . . . 1 9 5 R e f e r e n c e s . . . . . . . . . . . . . . . 1 9 7

A Study of Non-specific Complement-fixation with particular reference to the Interaction of Normal Serum and certain Non-antigenic substances