Open AccessConstruction of a fusion protein carrying antigenic determinants of enteric clostridial toxins
Author(s) -
Belyi Iouri F,
Varfolomeeva Nina A
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00560-3
Subject(s) - clostridium perfringens , microbiology and biotechnology , enterotoxin , biology , clostridium difficile , fusion protein , antigen , virulence , clostridium , virology , escherichia coli , bacteria , gene , biochemistry , genetics , recombinant dna , antibiotics
Abstract Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression of ToxA, ToxB and Cpe fragments either as individual components or as a hybrid multidomain (ToxA‐ToxB‐Cpe) protein. Rabbit monospecific sera raised against individual peptides reacted with the chimeric product indicating that the corresponding antigenic determinants were correctly expressed on the hybrid molecule. Furthermore, mice immunized with the fusion protein produced antibodies specific to each of the three separate components. These data suggest that the constructed three‐domain molecule could be used in future studies for development of a vaccine against enteric clostridial diseases.