Open AccessPurification of bacteriocin AS‐48 from an Enterococcus faecium strain and analysis of the gene cluster involved in its production 1
Author(s) -
Folli Claudia,
Ramazzina Ileana,
Arcidiaco Patrizia,
Stoppini Monica,
Berni Rodolfo
Publication year - 2003
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/s0378-1097(03)00176-9
Subject(s) - bacteriocin , enterococcus faecium , gene cluster , plasmid , biology , gene , coding region , structural gene , enterococcus faecalis , sequence analysis , microbiology and biotechnology , genetics , escherichia coli , bacteria
Abstract The cyclic bacteriocin AS‐48 has previously been shown to be produced by Enterococcus faecalis strains. A bacteriocin has been purified from an E. faecium strain ( E. faecium 7C5), and it has been found to possess molecular mass, cyclization and amino acid sequence typical of bacteriocin AS‐48. In addition to the structural gene as‐48A, the sequence analysis of the AS‐48 gene cluster present in E. faecium 7C5 has revealed the presence of several putative coding regions presumably involved in bacteriocin production and immunity. The results of DNA hybridization assays have indicated that the AS‐48 gene cluster and the gene pd78 are present on the same plasmid, possibly the pPD1 plasmid, in E. faecium 7C5.