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ObjectiveIn the Tzu Chi Taiwanese Bone Marrow Donor Registry individuals carrying the human leukocyte antigen (HLA)-C*03:77 allele and -DQB1*03:13 allele were registered. Here we report the confirmed DNA sequences of C*03:77 and DQB1*03:13 and the probable HLA haplotypes deduced in association with C*03:77 (i.e., HLA-A*02-B*40-C*03:77) and DQB1*03:13 (i.e., HLA-A*11:01-B*51:01-C*14:02-DRB1*11:01-DQB1*03:13) in Taiwanese.Materials and MethodsA sequence-based typing (SBT) method was used to confirm the low incidence alleles observed. Polymerase chain reaction was carried out to amplify exons 2 and 3 of the HLA-C locus with group-specific primer sets. For HLA-DQB1 allelic typing, six group-specific primer sets were used in the SBT procedure. Amplicons were sequenced using the Big Dye Terminator Cycle Sequencing Ready Reaction Kit (versions 3.1; Applied Biosystems, Foster City, CA, USA) in both directions according to the manufacturer's protocols.ResultsWe confirmed the DNA sequence of C*03:77, which is identical to the sequence of C*03:04:01:01 in exons 2 and 3 except for the nucleotide at position 527 (A→T). The nucleotide substitution caused an amino acid replacement at residue (codon) 152 (E→V). Similarly, our SBT confirmed that the DNA sequence of DQB1*03:13 is analogous to the sequence of DQB1*03:01:01:01 in exons 2 and 3, except for the nucleotide at residue 296 (T→A). The nucleotide variation caused one amino acid exchange at residue (codon) 67 (V→D).ConclusionsThe Taiwanese ethnicity of our donors identified in this study completes the ethnicity background for C*03:77 and DQB1*03:13 alleles listed on the IMGT/HLA Database and provides a strategy for marrow donor registry search coordinators to screen for unrelated HLA matched hematopoietic stem cell donors for blood disease patients bearing C*03:77 or DQB1*03:13

Recognition of HLA-A*11:01-B*51:01-C*14:02-DRB1*11:01-DQB1*03:13 and HLA-A*02-B*40-C*03:77-DRB1*14 haplotypes restricted to Taiwanese

Recognition of HLA-A11:01-B51:01-C14:02-DRB111:01-DQB103:13 and HLA-A02-B40-C03:77-DRB1*14 haplotypes restricted to Taiwanese