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The present study focuses on the standardization of the supercritical fluid extraction of the Bhut Jolokia ( Capsicum chinense)  which is abundantly grown in the north eastern region of India. The effect of process parameters pressure (75–225 bar), temperature (40–60 °C) and time (30–90 min) of oleoresin extraction process was studied. The standardized condition to obtain maximum extraction of Bhut Jolokia oleoresin was found to be 207 bar, 60 °C and 73 min. The oleoresin extract was analyzed for its antimicrobial, antioxidant and total phenolics content. Four strains of bacteria namely  Escherichia coli  (ATCC -11,229),  Bacillus subtilis  (ATCC- 11,774),  Salmonella typhimurium  (ATCC- 14,028) and  Staphylococcus aureus  (12,600) were used for the antimicrobial assay. It was observed that the highest inhibition was seen against  E. coli , moderate inhibition was seen against  S.aureus  and  S. typhi  and partial/no zone of inhibition was observed against  B. subtilis . The extract of Bhut Jolokia oleoresin showed radical scavenging activity of 58.6 ± 3.86% and total phenolics content of 4250 ± 2.26 mg GAE/100 g sample indicating Bhut Jolokia oleoresin as a good antioxidant and is also a good source of phenolic compounds.

Supercritical fluid extraction of Bhut Jolokia oleoresin and its quality analysis